Ponceau S
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Identifiers | |
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6226-79-5 | |
ChEBI | CHEBI:90319 |
Jmol 3D model | Interactive image |
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Properties | |
C22H16N4O13S4 | |
Molar mass | 672.63 g·mol−1 |
Vapor pressure | {{{value}}} |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references | |
Ponceau S, Acid Red 112, or C.I. 27195 is a sodium salt of a diazo dye of a light red color, that may be used to prepare a stain for rapid reversible detection of protein bands on nitrocellulose or polyvinylidene fluoride (PVDF) membranes (Western blotting), as well as on cellulose acetate membranes.[1] A Ponceau S stain is useful because it does not appear to have a deleterious effect on the sequencing of blotted polypeptides and is therefore one method of choice for locating polypeptides on Western blots for blot-sequencing.[1] It is also easily reversed with water washes, facilitating subsequent immunological detection.[1] The stain can be completely removed from the protein bands by continued washing.[1] Common stain formulations include 0.1% (w/v) Ponceau S in 5% acetic acid or 2% (w/v) Ponceau S in 30% trichloroacetic acid and 30% sulfosalicylic acid.[2]
See also
- Western blotting
- Coomassie Brilliant Blue
- Ponceau family of Azo dyes