Azotobacter vinelandii
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A. vinelandii
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| Azotobacter vinelandii |
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Azotobacter vinelandii is gram-negative diazotroph that can fix nitrogen while grown aerobically. It is a genetically tractable system that is used to study nitrogen fixation. These bacteria are easily cultured and grown.
A. vinelandii is a free-living N2 fixer known to produce many phytohormones and vitamins in soils. It produces fluorescent pyoverdine pigments.[2]
The nitrogenase holoenzyme of Azotobacter vinelandii has been characterised via x-ray crystallography in both ADP tetrafluoroaluminate-bound[3] and MgATP-bound[4] states. The enzyme possesses molybdenum iron-sulfido cluster cofactors (FeMoco) as active sites, each bearing 2 pseudo-cubic iron-sulfido structures.
References
- ↑ Lua error in package.lua at line 80: module 'strict' not found.
- ↑ Menhart, N., Thariath, A., Viswanatha, T. (1991) Characterization of the pyoverdines of Azotobacter vinelandii ATCC 12837 with regard to heterogeneity. Biology of Metals 4(4):223-32
- ↑ Schindelin, H., Kisker, C., Schlessman, J.L., Howard, J.B., Rees, D.C. (1997) Structure of ADP x AIF4(-)-stabilized nitrogenase complex and its implications for signal transduction. Nature 387: 370-376
- ↑ Chiu, H., Peters, J.W., Lanzilotta, W.N., Ryle, M.J., Seefeldt, L.C., Howard, J.B., Rees, D.C. (2001) MgATP-Bound and nucleotide-free structures of a nitrogenase protein complex between the Leu 127 Delta-Fe-protein and the MoFe-protein. Biochemistry 40: 641-650
External links
- Azotobacter vinelandii Genome Project
- Current research on Azotobacter vinelandii at the Norwich Research Park
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